Diclofop-Methyl Increases the Proton Permeability of Isolated Oat-Root Tonoplast
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چکیده
منابع مشابه
Effects of diclofop and diclofop-methyl on the membrane potentials of wheat and oat coleoptiles.
Electrophysiological measurements were made on the mesophyll cells of wheat (Triticum aestivum L. cv Waldron) and oat (Avena sativa L. cv Garry) coleoptiles treated either with the herbicide diclofop-methyl (methyl 2-(4-(2',4'-dichlorophenoxy)phenoxy)propanoate), or it's primary metabolite diclofop, (2-(4-(2',4'-dichlorophenoxy)phenoxy)-propanoic acid). Application of a 100 micromolar solution ...
متن کاملEffects of diclofop and diclofop-methyl on membrane potentials in roots of intact oat, maize, and pea seedlings.
Growth and electrophysiological studies in roots of intact diclofop-methyl susceptible and resistant seedlings were conducted to test the hypothesis that the herbicide acts primarily as a proton ionophore. The ester formulation of diclofop, at 0.2 micromolar, completely inhibited root growth in herbicide-susceptible oat (Avena sativa L.) after a 96 hour treatment, but induced only a delayed tra...
متن کاملLysophosphatidylcholine stimulates ATP dependent proton accumulation in isolated oat root plasma membrane vesicles.
Lysophosphatidylcholine at concentrations of 30 micromolar stimulated the rate of MgATP-dependent H(+)-accumulation in oat (Avena sativa L. cv Rhiannon) root plasma membrane vesicles about 85% while the passive permeability of H(+) was unchanged. Activation was dependent on chain length, degree of saturation, and head group of the lysophospholipid. A H(+)-ATPase assay was developed that allowed...
متن کاملPotential-dependent anion transport in tonoplast vesicles from oat roots.
Potential-dependent anion movement into tonoplast vesicles from oat roots (Avena sativa L. var Lang) was monitored as dissipation of membrane potentials (Deltapsi) using the fluorescence probe Oxonol V. The potentials (positive inside) were generated with the H(+)-pumping pyrophosphatase, which is K(+) stimulated and anion insensitive. The relative rate of DeltaPsi dissipation by anions was use...
متن کاملATP synthesis and pyrophosphate-driven proton transport in tonoplast-enriched vesicles isolated from Catharanthus roseus
In the presence of PP, as an energy source, the tonoplast-bound inorganic pyrophosphatase from Catharanthus roseus cells is able to create a proton-gradient which can drive the synthesis of ATP from ADP and Pi. ATP synthesis is linked to the pH-gradient dissipation as monitored by the recovery of the fluorescence intensity of quinacrine and by the amount of synthesized ATP measured by the biolu...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1989
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.91.2.756